Structural Biochemistry and Hands-on course of Biochemistry Methodologies
COURSE CONTENT
Protein purification methods
Introduction to protein structure and protein folding
General consideration and techniques to monitor protein structure
Electrophoretic methods of proteins (SDS-PAGE, IEF, 2D-electrophoresis)
Fingerprinting analysis of a protein
Separation of a complex peptide mixture by reversed-phase chromatography
Determination of the protein primary sequence by Edman Degradation
Mass spectrometry of proteins: theory and applications
Spectroscopic characterization of protein by circular dichroism, fluorescence and Fourier-transformed Infrared
Chemical and physico-chemical characterization of a recombinant proteins
Proteomics
LEARNING OUTCOME
The aim of the course is the learning of recent techniques of purification and characterization of proteins and peptides. This course is designed to let students explore some of the many biochemical methods that are utilized for elucidating structural and functional properties of proteins. The practical laboratory is designed to introduce and emphasize important techniques in biochemistry, including, electrophoresis, liquid chromatography, mass spectrometry and biospectroscopy. Some aspects of proteomics are introduced
CREDITS 5+6
CONTACT INFORMATION
Patrizia Polverino de Laureto
CRIBI
Via Giuseppe Colombo 3
35131 Padova
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Angelo Fontana
CRIBI, Viale G. Colombo 3
35121 Padova
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