Course name: Clinical Biochemistry
COURSE CONTENT
The clinical laboratory section includes a discussion of specimen collection, sources of error and variability (pre-analytical, analytical and biological variability),reference ranges, quality assurance.
Methods of clinical chemistry: Centrifugation, chromatography, protein and DNA electrophoresis. Spectroscopic techniques: absorbtion, fluorescence, flame, NMR and MS spectroscopy.
Diagnostic enzymology. Enzymes as reagents. Diagnostic considerations on lactate dehydrogenase, creatine kinase, alanine and aspartate transaminases, alkaline phosphatase. Enzymes in therapy. Significance of the variations of several parameters during common disease, such as diabetes and myocardium infarct.
Immunochemical tecniques: immunoelectrophoresis anche the use of the antibodies. Immunoassays: RIA, ELISA, IRMA, EMIT, FPIA.
Elements of Molecular Biology. Restriction enzymes. Plasmids. Recombinant protein production. Construction of a gene probe. DNA hybridation. Polymerase chain reaction (PCR). DNA sequencing. Clinical applications. DNA finger printing in forensic medicine. Prenatal diagnosis to detect genetic diseases.
Fat-soluble vitamins (A, D, E, K) and Vitamin A in the mechanism of vision. Water soluble vitamins and coenzymes: vitamin C, thiamine diphosphate, FMN, FAD, NAD, NADP, pyridoxal phosphate, coenzime A, tetrahydrofolic acid, cobamide coenzymes. Lipoic acid and biotin. Carnitine. Cytochrome P450. Nitric oxide synthase.
LEARNING OUTCOME
At the end of the course students will understand the medical, scientific and technological principles of Clinical Biochemistry and its interrelationship with other disciplines.
CREDITS: 6
CONTACT INFORMATION
Marcella Canton
Department of Biomedical Sciences,
University of Padova, Italy
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